primary antibodies against runx2  (Bioss)

Journal: Polymers

Article Title: An Electrospun DFO-Loaded Microsphere/SAIB System Orchestrates Angiogenesis–Osteogenesis Coupling via HIF-1α Activation for Vascularized Bone Regeneration

doi: 10.3390/polym17111538

Figure Lengend Snippet: Osteogenic functional analysis of BMSCs in an endothelial co-culture system. ( A ) Wound-healing process of BMSCs and semiquantitative analysis (wound width). ( B ) Quantitative analysis of the wound-healing process at 12 h and 24 h. ( C ) Migration ability of BMSCs evaluated by the Transwell system. ( D ) Quantitative analysis of the migrated BMSCs. ( E ) ALP staining of BMSCs at day 7. ( F ) ARS staining of BMSCs at day 21. ( G ) Quantitative analysis of Runx2 fluorescence. ( H ) Representative Runx2 fluorescence image of BMSCs. ( I ) Expression of proteins Runx2 and Col1a1 in BMSCs. ( J , K ) Quantitative analysis of the relative Runx2 and Col1a1 expression. Error bars, mean ± standard deviation; * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001 ( n = 3).

Article Snippet: A primary antibody against Runx2 (rabbit monoclonal, 1:200; Servicebio, Wuhan, China) was applied overnight at 4 °C, followed by incubation with an Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody (2 h, RT, 1:500; Servicebio, Wuhan, China).

Techniques: Functional Assay, Co-Culture Assay, Migration, Staining, Fluorescence, Expressing, Standard Deviation

Journal: Polymers

Article Title: An Electrospun DFO-Loaded Microsphere/SAIB System Orchestrates Angiogenesis–Osteogenesis Coupling via HIF-1α Activation for Vascularized Bone Regeneration

doi: 10.3390/polym17111538

Figure Lengend Snippet: Bone regeneration efficacy in calvarial defects. ( A ) Establishment of a rat calvarial-defect model (defect size = 5 mm). ( B ) Rat skull 3D rendering at 8 weeks. ( C ) H&E staining and Masson‘s trichrome staining at 8 weeks (NB: new bone; DM: defect margin; OB: osteoblast; CT: connective tissue; BV: blood vessels). ( D ) Paired difference in BV/TV. ( E ) Paired difference in relative new-bone area. ( F ) Paired difference in BMD. ( G ) Immunohistochemical staining of Runx2 and Col1a1. ( H ) Quantitative evaluation of the expression of Runx2 and Col1a1. Error bars, mean ± standard deviation; ** p < 0.01; **** p < 0.0001 ( n = 5).

Article Snippet: A primary antibody against Runx2 (rabbit monoclonal, 1:200; Servicebio, Wuhan, China) was applied overnight at 4 °C, followed by incubation with an Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody (2 h, RT, 1:500; Servicebio, Wuhan, China).

Techniques: Staining, Immunohistochemical staining, Expressing, Standard Deviation

Journal: International Journal of Nanomedicine

Article Title: Anti-Infection Efficacy, Osteogenesis Potential, and Biocompatibility of 3D Printed PLGA/Nano-Hydroxyapatite Porous Scaffolds Grafted with Vancomycin/DOPA/rhBMP-2 in Infected Rabbit Bone Defects

doi: 10.2147/IJN.S514978

Figure Lengend Snippet: Immunohistochemical analysis of osteogenesis-related proteins in IBD rabbit models. ( A ) Representative immunohistochemical staining images showing RUNX2 and OCN expression in bone tissues of IBD rabbit models after scaffold implantation at 12 weeks post-surgery. ( B and C ) Quantification of RUNX2-positive and OCN-positive cells in each group. **p < 0.01, ***p < 0.001 versus the PLGA/n-HA group; ### p < 0.001 versus the PLGA/n-HA/VAN group.

Article Snippet: After 1 h of non-specific binding blockage with 10% goat serum, the sections were incubated with the primary antibodies against RUNX2 (#A2851; 1:100; ABclonal, Wuhan, China) and OCN (#HZK-11654; 1:100; Huzhen, Shanghai, China) at 4 °C overnight and washed three times with PBS.

Techniques: Immunohistochemical staining, Staining, Expressing